Conception of a New Approach to Detection of Lymph Humor Factor in Thoracic Duct Lymph of Rabbits

There is lymph humor/lymphocytopoietic factor (LHF) in various tissues. However, a definite substance or a definite combination of any substances of LHF in thoracic duct lymph remains unknown. Justification for the conception of a new approach to revealing of LHF composition in native thoracic duct lymph is based on a discovery of mitoses in native thoracic duct lymph of normal immature rabbits. No mitoses are strongly detected in native thoracic duct lymph of normal mature rabbits. The aim of the work is to justify a new approach to detection of definite LHF. Two groups of anesthetized normal Chinchilla rabbit males should be formed: group 15 or more adult rabbits, group 2 5 or more immature rabbits. The conception of the new approach suggests a certain succession of stages. 1. To obtain native lymph from cistern chyle of thoracic duct with a glass micropipette (Patent No 1495076, Russia). 2. To make lymph plasma from thoracic duct lymph of rabbits of group 1 and group 2. 3. To find a qualitative/quantitative substances composition of lymph plasma in group 1 and group 2. 4. To find the difference between the lymph plasma composition in group 1 and group 2. Consequently, the difference will be the sought-for LHF. The conception of the new methodical approach can be used to study different features of thoracic duct lymph, particularly cells division, identification of lymph humor/lymphocytopoietic factor, that can play a key role in access to cell dividing mechanism and it is the way to elaborate new cancer drugs.